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phosphorylated p pparγ  (Bioss)


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    Structured Review

    Bioss phosphorylated p pparγ
    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, <t>p-PPARγ</t> and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, <t>phosphorylated;</t> PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
    Phosphorylated P Pparγ, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+ppar%CE%B3+ser112/PPAR+Gamma+(ser112)+Polyclonal+Antibody/pmc11419410-126-21-27
    Average 93 stars, based on 14 article reviews
    phosphorylated p pparγ - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells"

    Article Title: Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2024.5690

    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.
    Figure Legend Snippet: Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Techniques Used: Expressing, Western Blot

    Related Articles

    Western Blot:

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    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.

    Expressing:

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization
    Article Snippet: PVDF membranes were blocked at room temperature for 20 min using a rapid sealing solution. .. The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal). .. Visualization reagent (PL101, Shenzhen SunView technology Co., Ltd.) was used, and all blotted

    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.

    Immunofluorescence:

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization
    Article Snippet: PVDF membranes were blocked at room temperature for 20 min using a rapid sealing solution. .. The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal). .. Visualization reagent (PL101, Shenzhen SunView technology Co., Ltd.) was used, and all blotted

    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.

    Staining:

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization
    Article Snippet: PVDF membranes were blocked at room temperature for 20 min using a rapid sealing solution. .. The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal). .. Visualization reagent (PL101, Shenzhen SunView technology Co., Ltd.) was used, and all blotted

    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.

    Saline:

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization
    Article Snippet: PVDF membranes were blocked at room temperature for 20 min using a rapid sealing solution. .. The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal). .. Visualization reagent (PL101, Shenzhen SunView technology Co., Ltd.) was used, and all blotted

    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.

    Knock-Out:

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization
    Article Snippet: PVDF membranes were blocked at room temperature for 20 min using a rapid sealing solution. .. The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal). .. Visualization reagent (PL101, Shenzhen SunView technology Co., Ltd.) was used, and all blotted

    Article Title: Adropin attenuates pancreatitis‑associated lung injury through PPARγ phosphorylation‑related macrophage polarization.
    Article Snippet: The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.The PVdF membranes were blocked at room temperature for 20 min using a rapid sealing solution.. The primary antibodies were used at 4˚C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5‑72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. Ac001, ABclonal); peroxisome prolifer‑ ator‑activated receptor γ (PPARγ; 1:1,000, cat. no. bsm‑52220R, BIOSS); p‑PPARγ Ser112 (1:1,000, cat. no. bs‑3737R, BIOSS); p‑PPARγ Ser273 (1:1,000, cat. no. bs‑2875R, BIOSS), caspase‑3 (1:1,000, cat. no. Yc0006, ImmunoWay Biotechnology company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).. Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.Visualization reagent (PL101, Shenzhen SunView technology co., Ltd.) was used, and all blotted bands were analyzed using ImageJ 1.48 software (National Institutes of Health), and the intensity values were normalized to GAdPH.



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    Image Search Results


    Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Journal: International Journal of Oncology

    Article Title: Cinobufagin inhibits M2-like tumor-associated macrophage polarization to attenuate the invasion and migration of lung cancer cells

    doi: 10.3892/ijo.2024.5690

    Figure Lengend Snippet: Effects of CB on the expression of macrophage polarization-related proteins. (A) Gel blots showing protein expression of Arg-1, iNOS, p-PPARγ and PPARγ in cells with different treatment groups. (B-D) Quantification of (B) Arg-1, (C) iNOS and (D) p-PPARγ based on the western blot assay (n=3). ** P<0.01 compared with M0 group; # P<0.05, ## P<0.01 and $$ P<0.01 compared with M2 group; ▲ P<0.05 and ▲▲ P<0.01 compared with M2 + RSG group. CB, cinobufagin; Arg-1, arginase 1; iNOS, inducible nitric oxide synthase; p-, phosphorylated; PPARγ, peroxisome proliferator-activated receptor γ; RSG, rosiglitazone; ns from M2 group, not statistically significant compared with M0 group; ns from M2 + RSG group, not statistically significant compared with M2 group.

    Article Snippet: After that, primary antibodies against Arg-1 (1:1,000; cat. no. bs-23837R; BIOSS), inducible nitric oxide synthase (iNOS; 1:1,000; cat. no. bs-0162R; BIOSS); phosphorylated (p)-PPARγ (1:1,000; cat. no. bs-3737R; BIOSS), vimentin (1:1,000; cat. no. bsm-33170M; BIOSS), E-cadherin (1:1,000; cat. no. bs-1519R; BIOSS), N-cadherin (1:1,000; cat. no. bs-20623R; BIOSS) and GAPDH (1:3,000; cat. no. 60004-1-Ig; Proteintech Group, Inc.) were incubated with the membrane overnight at 4°C, followed by washing with Tris-buffered saline containing 0.05% Tween 20 and incubation with horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG (1:10,000; cat. no. SA00001-1) and goat anti-rabbit IgG (1:10,000; SA00001-2; both from Proteintech Group, Inc.) at room temperature for 1 h. The western blot bands were visualized using an ECL kit (cat. no. PE0010; Beijing Solarbio Science & Technology Co., Ltd.).

    Techniques: Expressing, Western Blot

    Excessive M1 macrophage polarization is observed in the Adro-KO + L-Arg group. (A) Western blot analysis of lung tissue form the Adro-KO + L-Arg group. (B) Co-expression of CD68 and iNOS in lung tissue in L-Arg group determined using immunofluorescence. (C) Co-expression of CD68 and CD206 of lung tissue in the L-Arg group determined using immunofluorescence. (D) iNOS mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (E) CD68 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥7). (F) CD163 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (G) Arg-1 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (H) Quantitative analysis of immunofluorescence (CD68 + iNOS) staining (n≥5). (I) Quantitative analysis of immunofluorescence (CD68; CD206) staining in macrophages (n≥9). (J) Quantitative analysis of the western blots (PPARγ; n≥6); (K) Quantitative analysis of the western blots (PPARγ Ser273; n≥6). (L) Quantitative analysis of the western blots (PPARγ Ser112; n≥6). * P<0.05. L-Arg, L-arginine; NS, normal saline; Adro-KO, adropin knockout; iNOS, inducible nitric oxide synthase.

    Journal: International Journal of Molecular Medicine

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization

    doi: 10.3892/ijmm.2023.5298

    Figure Lengend Snippet: Excessive M1 macrophage polarization is observed in the Adro-KO + L-Arg group. (A) Western blot analysis of lung tissue form the Adro-KO + L-Arg group. (B) Co-expression of CD68 and iNOS in lung tissue in L-Arg group determined using immunofluorescence. (C) Co-expression of CD68 and CD206 of lung tissue in the L-Arg group determined using immunofluorescence. (D) iNOS mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (E) CD68 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥7). (F) CD163 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (G) Arg-1 mRNA level in lung tissue from the Adro-KO + L-Arg group (n≥8). (H) Quantitative analysis of immunofluorescence (CD68 + iNOS) staining (n≥5). (I) Quantitative analysis of immunofluorescence (CD68; CD206) staining in macrophages (n≥9). (J) Quantitative analysis of the western blots (PPARγ; n≥6); (K) Quantitative analysis of the western blots (PPARγ Ser273; n≥6). (L) Quantitative analysis of the western blots (PPARγ Ser112; n≥6). * P<0.05. L-Arg, L-arginine; NS, normal saline; Adro-KO, adropin knockout; iNOS, inducible nitric oxide synthase.

    Article Snippet: The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Saline, Knock-Out

    Adropin exogenous supplement induces M2 macrophage polarization. (A) Western blot analysis of lung tissue from the Adro-KO + L-Arg + Adro (34−76) group. (B) Quantitative analysis of the western blots (PPARγ, PPARγ Ser112, PPARγ Ser273) (n≥5). (C) Co-expression of CD68 and iNOS in lung tissue from the Adro-KO + L-Arg+Adro (34−76) group. (D) Co-expression of CD68 and iNOS in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group. (E) Ratio of CD68/DAPI of lung in Adro-KO+ L-Arg+Adro (34−76) group(n≥3); (F) The ratio of iNOS/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (G) Ratio of iNOS/CD68 in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (H) Ratio of CD68/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (I) Ratio of CD206/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (J) Ratio of CD206/CD68 in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (K) CD163 mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (L) Arg-1 mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (M) iNOS mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (N) CD86 mRNA level level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). * P<0.05 and *** P<0.001. L-Arg, L-arginine; Adro-KO, adropin knockout; NS, normal saline; iNOS, inducible nitric oxide synthase; Arg-1, arginase 1.

    Journal: International Journal of Molecular Medicine

    Article Title: Adropin attenuates pancreatitis-associated lung injury through PPARγ phosphorylation-related macrophage polarization

    doi: 10.3892/ijmm.2023.5298

    Figure Lengend Snippet: Adropin exogenous supplement induces M2 macrophage polarization. (A) Western blot analysis of lung tissue from the Adro-KO + L-Arg + Adro (34−76) group. (B) Quantitative analysis of the western blots (PPARγ, PPARγ Ser112, PPARγ Ser273) (n≥5). (C) Co-expression of CD68 and iNOS in lung tissue from the Adro-KO + L-Arg+Adro (34−76) group. (D) Co-expression of CD68 and iNOS in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group. (E) Ratio of CD68/DAPI of lung in Adro-KO+ L-Arg+Adro (34−76) group(n≥3); (F) The ratio of iNOS/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (G) Ratio of iNOS/CD68 in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (H) Ratio of CD68/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (I) Ratio of CD206/DAPI in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (J) Ratio of CD206/CD68 in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥3). (K) CD163 mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (L) Arg-1 mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (M) iNOS mRNA level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). (N) CD86 mRNA level level in lung tissue from the Adro-KO + L-Arg + Adro (34−76) group (n≥6). * P<0.05 and *** P<0.001. L-Arg, L-arginine; Adro-KO, adropin knockout; NS, normal saline; iNOS, inducible nitric oxide synthase; Arg-1, arginase 1.

    Article Snippet: The primary antibodies were used at 4°C overnight, and the antibody included Adropin (1:1,000, cat. no. PA5-72781, Thermo Fisher Scientific, Inc.); GADPH (1:10,000; cat. no. AC001, ABclonal); peroxisome proliferator-activated receptor γ (PPARγ; 1:1,000, cat. no. bsm-52220R, BIOSS); p-PPARγ Ser112 (1:1,000, cat. no. bs-3737R, BIOSS); p-PPARγ Ser273 (1:1,000, cat. no. bs-2875R, BIOSS), caspase-3 (1:1,000, cat. no. YC0006, ImmunoWay Biotechnology Company) and PARP1 (1:1,000, cat. no. A0942, ABclonal).

    Techniques: Western Blot, Expressing, Knock-Out, Saline